Rhodopseudomonas globiformis Medium

[所属分类:培养基配方] [发布时间:2021-8-4] [发布人:网站管理员2] [阅读次数:] [返回]

Rhodopseudomonas globiformis Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1002.0mL:

Mannitol........................................................................................1.5g
KH 2 PO 4 .........................................................................................0.5g
Sodium gluconate .........................................................................0.5g
MgSO 4 ·7H 2 O............................................................................... 0.4g
NaCl..............................................................................................0.4g
NH 4 Cl ...........................................................................................0.4g
Yeast extract................................................................................0.25g
CaCl 2 ·2H 2 O.................................................................................0.05g
Ferric citrate solution.................................................................5.0mL
Na 2 S 2 O 3  solution........................................................................2.0mL
Biotin solution............................................................................1.0mL
p-Aminobenzoic acid solution...................................................1.0mL
Trace elements solution SL-6 ....................................................1.0mL
pH 4.9 ± 0.2 at 25°C
Ferric Citrate Solution:
Composition per 10.0mL:
Ferric citrate................................................................................0.01g
Preparation of Ferric Citrate Solution:  Add ferric citrate to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Na 2 S 2 O 3 Solution :
Composition per 10.0mL:
Na 2 S 2 O 3 ........................................................................................1.0g
Preparation of Na 2 S 2 O 3 Solution:  Add Na 2 S 2 O 3  to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
Biotin Solution:
Composition per 10.0mL:
Biotin .........................................................................................0.2mg
Preparation of Biotin Solution:  Add biotin to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
p-Aminobenzoic Acid Solution:
Composition per 10.0mL:
p-Aminobenzoic acid.................................................................1.0mg
Preparation of p-Aminobenzoic Acid Solution:  Add p-amin-
obenzoic acid to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C.
Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O .................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except Na 2 S 2 O 3  solu-
tion, ferric citrate solution, biotin solution, and p-aminobenzoic acid
solution, to distilled deionized water and bring volume to 993.0mL.
Mix thoroughly. Adjust pH to 4.9. Add 5.0mL of ferric citrate solution,
1.0mL of biotin solution, and 1.0mL of p-aminobenzoic acid solution.
Mix thoroughly. Distribute into screw-capped tubes or bottles. Auto-
clave for 15 min at 15 psi pressure–121°C. Allow to cool to room tem-
perature. Aseptically add 0.2mL of sterile Na 2 S 2 O 3  solution to each
100.0mL of medium. Mix thoroughly.
Use: For the cultivation and maintenance of Rhodopila globiformis.