Rhodopseudomonas blastica Medium

[所属分类:培养基配方] [发布时间:2021-8-4] [发布人:网站管理员2] [阅读次数:] [返回]

Rhodopseudomonas blastica Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sodium pyruvate...........................................................................1.5g
Sodium hydrogen malate..............................................................1.5g
Yeast extract..................................................................................1.0g
NH 4 Cl ...........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.4g
NaCl..............................................................................................0.4g
CaCl 2 ·2H 2 O ................................................................................0.05g
Sodium pyryuvate solution......................................................50.0mL
Sodium hydrogen malate solution...........................................50.0mL
Sodium phosphate buffer (0.1M, pH 6.8)................................50.0mL
pH 6.8 ± 0.2 at 25°C
Sodium Pyruvate Solution :
Composition per 50.0mL:
Sodium pyruvate...........................................................................1.5g
Preparation of Sodium Pyruvate Solution:  Add sodium pyru-
vate to distilled/deionized water and bring volume to 50.0mL. Mix
thoroughly. Filter sterilize.
Sodium Hydrogen Malate Solution :
Composition per 50.0mL:
Sodium hydrogen malate .............................................................1.5g
Preparation of Sodium Hydrogen Malate Solution:  Add sodi-
um hydrogen malate to distilled/deionized water and bring volume to
50.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components—except sodium pyru-
vate solution, sodium hydrogen malate solution, and sodium phosphate
buffer—to distilled/deionized water and bring volume to 850.0mL.
Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 6.8 with
KOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Filter sterilize the sodium pyruvate solution, sodium hydrogen
malate solution, and sodium phosphate buffer. Aseptically add 50.0mL
of sterile sodium pyruvate solution, 50.0mL of sodium hydrogen
malate solution, and 50.0mL of sodium phosphate buffer to cooled bas-
al medium. Mix thoroughly. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use:  For the cultivation and maintenance of Rhodopseudomonas blas-
tica and other Rhodopseudomonas species.