Rhodopila globiformis Medium (LMG Medium 81)

[所属分类:培养基配方] [发布时间:2021-8-4] [发布人:网站管理员2] [阅读次数:] [返回]

Rhodopila globiformis Medium
(LMG Medium 81)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1008.0mL:

Mannitol........................................................................................1.5g
Sodium gluconate .........................................................................0.5g
KH 2 PO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.4g
NH 4 Cl ...........................................................................................0.4g
NaCl..............................................................................................0.4g
Yeast extract................................................................................0.25g
CaCl 2 ·2H 2 O..............................................................................50.0mg
Ferric citrate solution.................................................................5.0mL
Na 2 SO 3 solution.........................................................................2.0mL
Trace elements solution .............................................................1.0mL
Biotin solution............................................................................1.0mL
p-Aminobenzoic acid solution...................................................1.0mL
pH 4.9 ± 0.2 at 25°C
Ferric Citrate Solution:
Composition per 100.0mL:
Ferric citrate..................................................................................0.1g
Preparation of Ferric Citrate Solution:  Add ferric citrate to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Trace Elements Solution:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
Na 2 MoO 4 ·2H 2 O.......................................................................30.0mg
MnCl 2 ·4H 2 O ............................................................................30.0mg
NiCl 2 ·6H 2 O..............................................................................20.0mg
CuCl 2 ·2H 2 O.............................................................................10.0mg
Preparation of Trace Elements Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Biotin Solution:
Composition per 100.0mL:
Biotin.........................................................................................2.0mg
Preparation of Biotin Solution:  Add biotin to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly.
p-Aminobenzoic Acid Solution:
Composition per 100.0mL:
p-Aminobenzoic Acid..............................................................10.0mg
Preparation of p-Aminobenzoic Acid Solution:  Add p-amin-
obenzoic acid to distilled/deionized water and bring volume to
100.0mL. Mix thoroughly.
Na 2 S 2 O 3 Solution:
Composition per 10.0mL:
Na 2 S 2 O 3 ........................................................................................1.0g
Preparation of Na 2 S 2 O 3 Solution:  Add Na 2 S 2 O 3  to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except Na 2 S 2 O 3  solu-
tion, ferric citrate solution, biotin solution, and p-aminobenzoic acid
solution, to distilled deionized water and bring volume to 1.0mL. Mix
thoroughly. Adjust pH to 4.9. Add 5.0mL of ferric citrate solution,
1.0mL of biotin solution, and 1.0mL of p-aminobenzoic acid solution.
Mix thoroughly. Distribute into screw-capped tubes or bottles. Auto-
clave for 15 min at 15 psi pressure–121°C. Allow to cool to room tem-
perature. Aseptically add 0.2mL of sterile Na 2 S 2 O 3  solution to each
100.0mL of medium. Mix thoroughly.
Use: For the cultivation and maintenance of Rhodopila globiformis
spp.