Rhodopila globiformis Medium

[所属分类:培养基配方] [发布时间:2021-8-4] [发布人:网站管理员2] [阅读次数:] [返回]

Rhodopila globiformis Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Mannitol........................................................................................1.5g
Sodium gluconate .......................................................................0.56g
KH 2 PO 4 .........................................................................................0.4g
NaCl..............................................................................................0.4g
MgCl 2 ·6H 2 O .................................................................................0.4g
NH 4 Cl ...........................................................................................0.4g
Na 2 S 2 O 3 ·5H 2 O..............................................................................0.2g
CaCl 2 ·2H 2 O.................................................................................0.05g
Ferric citrate...............................................................................5.0mg
VA vitamins ...............................................................................1.0mL
Trace elements solution SL-6 ....................................................1.0mL
pH 4.9 ± 0.2 at 25°C
VA Vitamins:
Composition per 500.0mL:
Nicotinamide.............................................................................0.175g
Thiamine·HCl .............................................................................0.15g
p-Aminobenzoic acid....................................................................0.1g
Biotin ..........................................................................................0.05g
Pyridoxine·2HCl.........................................................................0.05g
Calcium pantothenate .................................................................0.05g
Cyanocobalamin .......................................................................0.025g
Preparation of VA Vitamins: Add components to distilled/deion-
ized water and bring volume to 500.0mL. Mix thoroughly.
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O................................................................................0.03g
Na 2 MoO 4 ·H 2 O ............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 · 2H 2 O.................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 4.9.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use:  For the cultivation and maintenance of Rhodopila globiformis.