Rhizobium X Medium with Thiram

[所属分类:培养基配方] [发布时间:2021-8-4] [发布人:网站管理员2] [阅读次数:] [返回]

Rhizobium X Medium with Thiram

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Mannitol......................................................................................10.0g
Yeast extract..................................................................................1.0g
Soil extract.............................................................................200.0mL
Thiram solution........................................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Thiram Solution:
Composition per 10.0mL:
Thiram........................................................................................1.0mg
Ethanol, absolute......................................................................10.0mL
Preparation of Thiram Solution: Add thiram to 10.0mL of abso-
lute ethanol. Mix thoroughly. Filter sterilize.
Soil Extract:
Composition per 200.0mL:
African Violet soil.......................................................................77.0g
Na 2 CO 3  .........................................................................................0.2g
Preparation of Soil Extract: Add components to tap water and
bring volume to 200.0mL.
Preparation of Medium:  Add components, except thiram solution,
to distilled/deionized water and bring volume to 990.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°C. Aseptically add 10.0mL of sterile thi-
ram solution. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the cultivation and maintenance of Bradyrhizobium japoni-
cum, Rhizobium species, and Sinorhizobium xinjiangensis.