Rhizobium X Medium

[所属分类:培养基配方] [发布时间:2021-8-4] [发布人:网站管理员2] [阅读次数:] [返回]

Rhizobium X Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Mannitol......................................................................................10.0g
Yeast extract..................................................................................1.0g
Soil extract.............................................................................200.0mL
pH 7.2 ± 0.2 at 25°C
Soil Extract:
Composition per 200.0mL:
African Violet soil.......................................................................77.0g
Na 2 CO 3  .........................................................................................0.2g
Preparation of Soil Extract: Add components to tap water and
bring volume to 200.0mL. Autoclave for 60 min at 15 psi pressure–
121°C. Filter through Whatman #1 filter paper.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Bradyrhizobium japoni-
cum, Rhizobium species, and Sinorhizobium xinjiangensis.