Rhizoctonia Isolation Medium

[所属分类:培养基配方] [发布时间:2021-8-4] [发布人:网站管理员2] [阅读次数:] [返回]

Rhizoctonia Isolation Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................20.0g
K 2 HPO 4 .........................................................................................1.0g
KCl................................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.5g
NaNO 2 ..........................................................................................0.2g
FeSO 4 ·7H 2 O................................................................................0.01g
Dexon ® solution......................................................................10.0mL
Antibiotic solution...................................................................10.0mL
Gallic acid solution..................................................................10.0mL
Antibiotic Solution:
Composition per 10.0mL:
Chloramphenicol.........................................................................0.05g
Streptomycin...............................................................................0.05g
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Dexon ® Solution:
Composition per 10.0mL:
Dexon ® (Chemagro ® ) wettable powder.....................................0.09g
Preparation of Dexon ® Solution: Add Dexon ® to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Gallic Acid Solution:
Composition per 10.0mL:
Gallic acid.....................................................................................0.4g
Preparation of Gallic Acid Solution: Add gallic acid to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components—except Dexon ® solu-
tion, antibiotic solution, and gallic acid solution—to distilled/deion-
ized water and bring volume to 970.0mL. Mix thoroughly. Gently heat
and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 45°–50°C. Aseptically add sterile Dexon ® solution, sterile an-
tibiotic solution, and sterile gallic acid solution. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Rhizoctonia species.