Alkaliphilic Halomonas Medium (DSMZ Medium 1034)

[所属分类:培养基配方] [发布时间:2019-9-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alkaliphilic Halomonas Medium
(DSMZ Medium 1034)
Composition per liter:
Yeast extract ...............................................................................10.0g
Sodium citrate.............................................................................. 3.0g
MgSO 4 ·7H 2 O ........................................................................1.0g
Solution C..............................................................................100.0mL
Solution D................................................................................10.0mL
Solution A .................................................................................1.0mL
Solution B .................................................................................1.0mL
pH 9.0 ± 0.2 at 25°C
Solution A:
Composition per 10.0mL:
MnCl 2 ·4H 2 O ..................................................................... 3.6mg
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to room temperature.
Solution B:
Composition per 10.0mL:
FeSO 4 ·7H 2 O..................................................................................0.5g
Preparation of Solution B:  Add components to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to room temperature.
Solution C:
Composition per 100.0mL:
NaCl .........................................................................................100.0g
Preparation of Solution C:  Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to room temperature.
Solution D:
Composition per 10.0mL:
Na 2 CO 3 ................................................................................3.0g
Preparation of Solution D:  Add components to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to room temperature.
Preparation of Medium: Add components, except solutions A, B,
C, and D, to distilled/deionized water and bring volume to 890.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 25°C. Aseptically add solutions A,
B, C, and D. Mix thoroughly. The pH should be 9.0.
Use: For the maintenance or cultivation of alkaliphilic Halomonas
spp.