Alkaliphilic Methanogen Medium

[所属分类:培养基配方] [发布时间:2019-9-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alkaliphilic Methanogen Medium
Composition per liter:
NaCl............................................................................................15.0g
NaHCO 3 ......................................................................................10.0g
Methanol.......................................................................................5.0g
Na 2 CO 3 .........................................................................................4.0g
Na 2 S·9H 2 O....................................................................................1.0g
NH 4 Cl ...........................................................................................0.5g
Yeast extract..................................................................................0.5g
KH 2 PO 4 .........................................................................................0.3g
NiCl 2 ·6H 2 O................................................................................2.0mg
Resazurin...................................................................................0.5mg
Wolfe’s mineral solution..........................................................10.0mL
Selenite/tungstate solution.........................................................1.0mL
pH 9.2–9.4 at 25°C
Wolfe’s Mineral Solution:
Composition per liter:
MgSO 4 ·7H 2 O................................................................................3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·2H 2 O................................................................................0.5g
CoCl 2 ·6H 2 O..................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
© 2010 by Taylor and Francis Group, LLC
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78 Alkaliphilic Spirochete Medium
CaCl 2 ·2H 2 O...................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
NiCl 2 ·6H 2 O...............................................................................0.025g
KAl(SO 4 ) 2 ·12H 2 O.......................................................................0.02g
CuSO 4 ·5H 2 O...............................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·2H 2 O ..........................................................................0.01g
Na 2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with
KOH. Add remaining components. Add distilled/deionized water to
1.0L. Adjust pH to 6.8.
Selenite/Tungstate Solution:
Composition per liter:
NaOH............................................................................................0.5g
Na 2 WO 4 ·2H 2 O...........................................................................4.0mg
Na 2 SeO 3 ·5H 2 O...........................................................................3.0mg
Preparation of Selenite/Tungstate Solution:  Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium:  Prepare and dispense medium under
100% N 2 . Add components, except NaHCO 3 and Na 2 S·9H 2 O, to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gen-
tly heat and bring to boiling. Continue boiling for 5 min. Cool to room
temperature while sparging with 100% N 2 . Add NaHCO 3 and
Na 2 S·9H 2 O. Mix thoroughly. Anaerobically distribute into tubes. Au-
toclave for 15 min at 15 psi pressure–121°C. Adjust pH to 9.2–9.4.
Use: For the cultivation of Methanohalophilus zhilinae.