Alkaline Yeast Extract Malt Medium

[所属分类:培养基配方] [发布时间:2019-9-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alkaline Yeast Extract Malt Medium
Composition per liter:
Malt extract.................................................................................10.0g
Yeast extract..................................................................................4.0g
Glucose .........................................................................................4.0g
Na 2 CO 3 (10% solution)..........................................................100.0mL
pH 8.5–11.0 at 25°C
Preparation of Medium:  Add components, except Na 2 CO 3 , to dis-
tilled/deionized water and bring volume to 900.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Separately sterilize a 10% Na 2 CO 3 solution and aseptical-
ly add 100.0mL. Adjust pH, if necessary, to 8.5–11.0.
Use: For the cultivation of Nocardiopsis dassonvillei.