Alkaline Xylan Medium

[所属分类:培养基配方] [发布时间:2019-9-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alkaline Xylan Medium
Composition per 1001.0mL:
Xylan...........................................................................................10.0g
Yeast extract..................................................................................3.0g
NH 4 NO 3 ........................................................................................2.0g
K 2 HPO 4 .........................................................................................1.0g
Polypeptone™...............................................................................0.3g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ·2H 2 O...................................................................................0.1g
FeSO 4 ·7H 2 O...............................................................................5.0mg
MnSO 4 ·7H 2 O.............................................................................5.0mg
Resazurin ...................................................................................1.0mg
Titanium citrate solution............................................................1.0mL
Na 2 CO 3  (10% solution)...........................................................variable
pH 10.0 ± 0.2 at 25°C
Titanium Citrate Solution:
Composition per 50.0mL:
TiCl 2 ............................................................................................0.75g
Trisodium citrate.........................................................................2.58g
Preparation of Titanium Citrate Solution: Add components to
approximately 30.0mL of distilled/deionized water. Mix thoroughly.
Adjust pH to 7.0 with Na 2 CO 3 . Bring volume to 50.0mL with distilled/
deionized water.
Preparation of Medium: Prepare and dispense medium under
100% N 2 . Add components, except titanium citrate solution, to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gen-
tly heat and bring to boiling. Continue boiling for 3 min. Cool to room
temperature while sparging with 100% N 2 . Anaerobically distribute
into anaerobic tubes. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to room temperature. Adjust pH to 10.0 with filter-sterilized 10%
Na 2 CO 3  solution. Immediately prior to inoculation, reduce medium by
adding 1.0mL of titanium citrate solution per liter of medium.
Use: For the cultivation of Amphibacillus xylanus.