Alkalibacterium olivapovliticus Medium (DSMZ Medium 923)

[所属分类:培养基配方] [发布时间:2019-9-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alkalibacterium olivapovliticus Medium
(DSMZ Medium 923)
Composition per liter:
Yeast extract..................................................................................5.0g
Na glutamate.................................................................................1.0g
(NH 4 ) 2 SO 4 .....................................................................................1.0g
K 2 HPO 4 .......................................................................................0.15g
MgSO 4 ·7H 2 O............................................................................0.025g
Na 2 CO 3  solution.....................................................................100.0mL
pH 9.5 ± 0.2 at 25°C
Na 2 CO 3 Solution:
Composition per 100.0mL:
Na 2 CO 3 .......................................................................................10.0g
Preparation of Na 2 CO 3 Solution:  Add Na 2 CO 3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Add components, except Na 2 CO 3  solu-
tion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
25°C. Aseptically add 100.0mL sterile Na 2 CO 3  solution. Mix thor-
oughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Bacillus sp. and Alkalibacterium olivapov-
liticus (Alkalibacterium olivoapovliticus).