Alkaliflexus Medium (DSMZ Medium 1175)

[所属分类:培养基配方] [发布时间:2019-9-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alkaliflexus Medium
(DSMZ Medium 1175)
Composition per liter:
NH 4 Cl ..........................................................................................0.2g
MgCl 2 ·6H 2 O ...................................................................... 0.05g
KH 2 PO 4 .........................................................................................0.2g
Na 2 S·9H 2 O solution...............................................................100.0mL
Yeast extract...........................................................................100.0mL
Cellobiose solution ..................................................................50.0mL
Na 2 CO 3  solution.......................................................................50.0mL
NaHCO 3 solution.....................................................................50.0mL
pH 10.0 ± 0.2 at 25°C
Yeast Extract Solution:
Composition per 100.0mL:
Yeast extract .................................................................................0.2g
Preparation of Yeast Extract Solution:  Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Sparge with N 2 . Autoclave for 15 min at 15 psi pressure–121°C. Cool
to room temperature.
Cellobiose Solution:
Composition per 50.0mL:
Cellulobiose..................................................................................3.0g
Preparation of Cellobiose Solution:  Add components to dis-
tilled/deionized water and bring volume to 50.0mL. Mix thoroughly.
Sparge with N 2 . Autoclave for 15 min at 15 psi pressure–121°C. Cool
to room temperature.
Na 2 CO 3 Solution :
Composition per 50.0mL:
Na 2 CO 3 .........................................................................................7.4g
Preparation of Na 2 CO 3 Solution:  Add components to distilled/
deionized water and bring volume to 50.0mL. Mix thoroughly. Sparge
with N 2 . Autoclave for 15 min at 15 psi pressure–121°C. Cool to room
temperature.
NaHCO 3 Solution :
Composition per 50.0mL:
NaHCO 3 ......................................................................................18.5g
Preparation of NaHCO 3 Solution:  Add components to distilled/
deionized water and bring volume to 50.0mL. Mix thoroughly. Sparge
with N 2 . Autoclave for 15 min at 15 psi pressure–121°C. Cool to room
temperature.
Na 2 S·9H 2 O Solution :
Composition per 100.0mL:
Na 2 S·9H 2 O....................................................................................0.5g
Preparation of Na 2 S·9H 2 O Solution: Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Autoclave under 100% N 2 for 15 min at 15 psi pressure–121°C. Cool
to room temperature.
Preparation of Medium: Add components, except Na 2 S·9H 2 O so-
lution, cellobiose solution, and yeast extract solution, to distilled/de-
ionized water and bring volume to 750.0mL. Mix thoroughly. Gently
heat and bring to boiling. Boil for several minutes. Cool to room tem-
perature while sparging with N 2 . Add the Na 2 CO 3  solution and the
NaHCO 3  solution. The pH should be 10.0. Distribute into serum bottles
or Hungate tubes. Seal the tubes under N 2 . Autoclave for 15 min at 15
psi pressure–121°C. Cool to 25°C. Aseptically inject Na 2 S·9H 2 O , cel-
lobiose, and yeast extract solutions to give concentrations of 10%, 5%,
and 10%, respectively.
Use: For the maintenance or cultivation of Alkaliflexus spp.