Alkalibacterium olivapovliticus Agar (DSMZ Medium 923)

[所属分类:培养基配方] [发布时间:2019-9-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alkalibacterium olivapovliticus Agar
(DSMZ Medium 923)
Composition per liter:
Yeast extract..................................................................................5.0g
Na glutamate.................................................................................1.0g
(NH 4 ) 2 SO 4 ....................................................................................1.0g
K 2 HPO 4 .......................................................................................0.15g
MgSO 4 ·7H 2 O............................................................................0.025g
Agar solution .........................................................................400.0mL
Na 2 CO 3  solution.....................................................................100.0mL
pH 9.5 ± 0.2 at 25°C
Agar Solution:
Composition per 400.0mL:
Agar............................................................................................20.0g
Preparation of Agar Solution:  Add agar to distilled/deionized
water and bring volume to 400.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 55°C.
Na 2 CO 3 Solution:
Composition per 100.0mL:
Na 2 CO 3 .......................................................................................10.0g
Preparation of Na 2 CO 3 Solution:  Add NaHCO 3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 55°C.
Preparation of Medium: Add components, except agar solution
and Na 2 CO 3  solution, to distilled/deionized water and bring volume to
500.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 55°C. Aseptically add 100.0mL sterile Na 2 CO 3  solu-
tion. Mix thoroughly. Aseptically add 400.0mL sterile agar solution.
Mix thoroughly. Pour into Petri dishes or aseptically distribute into
sterile tubes.
Use: For the cultivation of Bacillus sp. and Alkalibacterium olivapov-
liticus (Alkalibacterium olivoapovliticus).