Nutrient Agar, Buffered

[所属分类:培养基配方] [发布时间:2021-6-22] [发布人:网站管理员2] [阅读次数:] [返回]

Nutrient Agar, Buffered

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Peptone..........................................................................................5.0g
NaCl..............................................................................................5.0g
Na 2 HPO 4 ·12H 2 O.........................................................................2.39g
Yeast extract..................................................................................2.0g
Beef extract...................................................................................1.0g
KH 2 PO 4 .......................................................................................0.45g
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Adjust pH to 6.8. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or
leave in tubes.
Use: For the cultivation and maintenance of Acidovorax avenae, Acido-
vorax avenae, Acidovorax delafieldii, Acidovorax facilis, Acidovorax kon-
jaci, Acidovorax temperans, Aminobacter aminovorans, Chryseomonas
luteola, Comamonas acidovorans, Comamonas testosteroni, Flavimonas
oryzihabitans, Flavobacterium breve, Flavobacterium mizutaii, Hydrog-
enoflava palleronii, Hydrogenophaga flava, Hydrogenophaga pseudo-
flava, Hydrogenophaga taeniospiralis, numerous Pseudomonas species,
Sphingobacterium multivorum, Sphingobacterium spiritivorum, Week-
sella virosa, Weeksella zoohelcum, and Moraxella atlantae.