Nutrient Agar, Alkaline (LMG Medium 53)

[所属分类:培养基配方] [发布时间:2021-6-22] [发布人:网站管理员2] [阅读次数:] [返回]

Nutrient Agar, Alkaline
(LMG Medium 53)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Peptone......................................................................................... 5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................2.0g
Lab-Lemco beef extract................................................................1.0g
pH 9.5–10.0 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 9.5–
10.0 with sterile Na 2 CO 3  solution .  Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Bacillus alcalophilus and
Bacillus cohnii.