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Nutrient Agar with Formate, Fumarate, and Horse Blood (LMG Medium 250)

[所属分类:培养基配方] [发布时间:2021-6-22] [发布人:网站管理员2] [阅读次数:] [返回]
Nutrient Agar with Formate, Fumarate,
and Horse Blood
(LMG Medium 250)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Peptone ........................................................................................ 5.0g
NaCl..............................................................................................5.0g
Fumaric acid .................................................................................3.0g
Sodium formate ............................................................................2.0g
Yeast extract..................................................................................2.0g
Lab-Lemco beef extract................................................................1.0g
Horse blood, sterile defibrinated..............................................50.0mL
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components, except horse blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Adjust pH to 7.2. Gently heat and bring to boiling. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°C. Aseptically add 50.0mL
sterile defibrinated horse blood. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Campylobacter rectus
and Campylobacter gracilis.
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