Nitrogen-Free Agar (Norris Agar)

[所属分类:培养基配方] [发布时间:2021-6-21] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrogen-Free Agar
(Norris Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
CaCO 3 ...........................................................................................1.0g
K 2 HPO 4 ........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
NaCl..............................................................................................0.2g
FeSO 4 ·7H 2 O .................................................................................0.1g
Na 2 MoO 4 ·2H 2 O .........................................................................5.0mg
Glucose solution ......................................................................50.0mL
Glucose Solution:
Composition per 100.0mL:
Glucose .......................................................................................20.0g
Preparation of Glucose Solution: Add 20.0g of glucose to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize. Warm to 50°C.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 950.0mL. Mix
thoroughly. Adjust pH to 7.2. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Asepti-
cally add 50.0mL of sterile glucose solution. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Azomonas agilis, Azoto-
bacter chroococcum, and Azotobacter vinelandii.