Nitrogen-Free Agar

[所属分类:培养基配方] [发布时间:2021-6-21] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrogen-Free Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
CaCO 3 ...........................................................................................1.0g
K 2 HPO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
NaCl..............................................................................................0.2g
FeSO 4 ·7H 2 O..................................................................................0.1g
Na 2 MoO 4 ·2H 2 O.........................................................................5.0mg
Glucose solution ......................................................................50.0mL
pH 7.0 ± 0.2 at 25°C
Glucose Solution:
Composition per 50.0mL:
Glucose.......................................................................................10.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 50.0mL. Mix thoroughly. Filter steril-
ize.
Preparation of Medium: Add components, except glucose solu-
tion and agar, to distilled/deionized water and bring volume to
950.0mL. Mix thoroughly. Add agar. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Aseptically add 50.0mL of sterile glucose solution. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Azomonas agilis, Azomonas insignis,
Azomonas macrocytogenes, Azorhizophilus paspali, Azotobacter bei-
jerinckii, Azotobacter chroococcum, Azotobacter vinelandii, Beijer-
inckia acida, Beijerinckia fluminensis, Beijerinckia indica, Beijer-
inckia mobilis, and Derxia gummosa.