Nitrogen-Fixing Marine Medium

[所属分类:培养基配方] [发布时间:2021-6-21] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrogen-Fixing Marine Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Noble agar...................................................................................10.0g
MgSO 4 ·7H 2 O..............................................................................0.04g
CaCl 2 ·2H 2 O ................................................................................0.02g
K 2 HPO 4 ·3H 2 O ............................................................................0.02g
Na 2 CO 3  .......................................................................................0.02g
Citric acid...................................................................................3.0mg
Ferric ammonium citrate............................................................3.0mg
Disodium potassium EDTA.......................................................0.5mg
Seawater.................................................................................750.0mL
Trace metals A-5 mix ................................................................1.0mL
pH 8.5 ± 0.2 at 25°C
Trace Metals A-5 Mix:
Composition per liter:
H 3 BO 3 .........................................................................................2.86g
MnCl 2 ·4H 2 O ...............................................................................1.81g
ZnSO 4 ·7H 2 O.............................................................................0.222g
CuSO 4 ·5H 2 O.............................................................................0.079g
Co(NO 3 ) 2 ·6H 2 O..........................................................................0.05g
Na 2 MoO 4 ·2H 2 O........................................................................0.039g
Preparation of Trace Metals A-5 Mix: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to glass-distilled water
and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Adjust pH to
8.5 with KOH. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use:  For the cultivation and maintenance of Anabaena species.