Alicyclobacillus Agar (DSMZ Medium 402)

[所属分类:培养基配方] [发布时间:2019-9-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alicyclobacillus Agar
(DSMZ Medium 402)
Composition per liter:
Glucose.........................................................................................5.0g
KH 2 PO 4 .........................................................................................3.0g
Yeast extract..................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................0.5g
CaCl 2 ·2H 2 O.................................................................................0.25g
(NH 4 ) 2 SO 4 ...................................................................................0.2g
Agar solution..........................................................................500.0mL
Trace elements solution SL-6 ....................................................1.0mL
pH 4.0 ± 0.2 at 25°C
Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O..................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O ......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O ................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Agar Solution:
Composition per 500mL:
Agar ............................................................................................15.0g
Preparation of Agar Solution: Add agar to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°C.
Preparation of Medium: Add components, except trace elements
solution SL-6 and agar solution, to distilled/deionized water and bring
volume to 499.0mL. Mix thoroughly. Adjust pH to 4.0. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 45°C. Aseptically add 1.0mL
of sterile trace elements solution SL-6 and 500.0mL agar solution. Mix
thoroughly. Pour into sterile Petri dishes or aseptically distribute into
sterile tubes or flasks.
Use:  For the cultivation and maintenance of Alicyclobacillus spp.,
Bacillus sp., and Bacillus naganoensis.