Alicyclobacillus acidoterrestris Medium (LMG Medium 141)

[所属分类:培养基配方] [发布时间:2019-9-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alicyclobacillus acidoterrestris Medium
(LMG Medium 141)
Composition per liter:
Agar............................................................................................30.0g
Glucose.........................................................................................5.0g
K 2 HPO 4 .........................................................................................3.0g
Yeast extract..................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................0.5g
CaCl 2 ·2H 2 O ................................................................................0.25g
(NH 4 ) 2 SO 4 ....................................................................................0.2g
Agar solution .........................................................................500.0mL
Trace elements solution.............................................................1.0mL
pH 4.0 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
CaCl 2 ·2H 2 O ................................................................................0.66g
Na 2 MoO 4 ·2H 2 O............................................................................0.3g
ZnSO 4 ·7H 2 O...............................................................................0.18g
CoCl 2 ·6H 2 O................................................................................0.18g
CuSO 4 ·5H 2 O...............................................................................0.16g
MnSO 4 ·4H 2 O..............................................................................0.15g
H 3 BO 3 ...........................................................................................0.1g
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Agar Solution:
Composition per 500.0mL:
Agar............................................................................................30.0g
Preparation of Agar Solution: Add agar to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Preparation of Medium: Add components, except agar solution, to
distilled/deionized water and bring volume to 500.0mL. Mix thorough-
ly. Adjust pH to 4.0 with H 2 SO 4 . Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C. Aseptically add 500.0mL agar
solution. Mix thoroughly. Aseptically pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the cultivation of Alicyclobacillus acidoterrestris.