Alicyclobacillus cycloheptanicus Agar (LMG Medium 174)

[所属分类:培养基配方] [发布时间:2019-9-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alicyclobacillus cycloheptanicus Agar
(LMG Medium 174)
Composition per 1001.0mL:
Solution A..............................................................................500.0mL
Agar solution..........................................................................500.0mL
Trace elements solution SL-6 ....................................................1.0mL
pH 4.0 ± 0.2 at 25°C
Solution A:
Composition per 500.0mL:
Yeast extract..................................................................................5.0g
Glucose .........................................................................................5.0g
K 2 HPO 4 .........................................................................................3.0g
MgSO 4 ·7H 2 O................................................................................0.5g
CaCl 2 ·2H 2 O.................................................................................0.25g
(NH 4 ) 2 SO 4 .....................................................................................0.2g
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Adjust to pH 4.0.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O................................................................................0.03g
Na 2 MoO 4 ·H 2 O............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 · 2H 2 O ................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4. Filter sterilize.
Agar Solution:
Composition per 500.0mL:
Agar............................................................................................30.0g
Preparation of Agar Solution: Add agar to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Preparation of Medium: Aseptically combine 500.0mL solution
A, 500.0mL sterile agar solution, and 1.0mL sterile trace elements so-
lution SL-6. Mix thoroughly. Aseptically pour into sterile Petri dishes
or distribute into sterile tubes.
Use: For the cultivation of Alicyclobacillus cycloheptanicus.