Alginate Utilization Medium

[所属分类:培养基配方] [发布时间:2019-9-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alginate Utilization Medium
Composition per liter:
Solution B..............................................................................500.0mL
Solution A..............................................................................400.0mL
Solution C..............................................................................100.0mL
Solution A:
Composition per 400.0mL:
Marine salts.................................................................................38.0g
Preparation of Solution A:  Add marine salts to distilled/deionized
water and bring volume to 400.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
Solution B:
Composition per 500.0mL:
Agar............................................................................................20.0g
Sodium alginate..........................................................................10.0g
Preparation of Solution B:  Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
Solution C:
Composition per 100.0mL:
Tris·HCl buffer..........................................................................0.067g
NaNO 3 ......................................................................................0.047g
Ferric EDTA ............................................................................66.5mg
Sodium glycerophosphate........................................................6.67mg
Thiamine·HCl ...........................................................................67.0μg
Vitamin B 12 .................................................................................1.3μg
Biotin........................................................................................0.67μg
Preparation of Solution C:  Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Aseptically combine solutions A, B, and
C. For liquid medium, omit agar from solution B.
Use: For the cultivation of microorganisms that can utilize alginate as
a carbon source. Growth on alginate (production of alginase) is a diag-
nostic test used in the differentiation of Vibrio species.