Alicyclobacillus acidoterrestris Agar

[所属分类:培养基配方] [发布时间:2019-9-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alicyclobacillus acidoterrestris Agar
Composition per 1001.0mL:
Solution A..............................................................................500.0mL
Solution C..............................................................................500.0mL
Solution B..................................................................................1.0mL
pH 4.0 ± 0.2 at 25°C
Solution A:
Composition per 500.0mL:
Glucose .........................................................................................5.0g
KH 2 PO 4 .........................................................................................3.0g
Yeast extract..................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................0.5g
CaCl 2 ·7H 2 O.................................................................................0.25g
(NH 4 ) 2 SO 4 .....................................................................................0.2g
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 4.0.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°–55°C.
Solution B:
Composition per liter:
MnCl 2 ·4H 2 O..................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O ......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O ................................................................................0.01g
Preparation of Solution B:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Warm
to 50°–55°C.
Solution C:
Composition per 500.0mL:
Agar ............................................................................................15.0g
Preparation of Solution C:  Add agar to distilled/deionized water
and bring volume to 500.0mL. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°–55°C.
Preparation of Medium: Aseptically combine 500.0mL of solu-
tion A, 1.0mL of solution B, and 500.0mL of solution C. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Alicyclobacillus acidot-
errestris .