Algal Proteose Agar

[所属分类:培养基配方] [发布时间:2019-9-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Algal Proteose Agar
Composition per liter:
Agar............................................................................................15.0g
Proteose peptone...........................................................................1.0g
Bristol's solution...........................................................................1.0L
Bristol's Solution:
Composition per 1000.1mL:
NaNO 3  solution...........................................................................10.0g
KH 2 PO 4  solution...........................................................................7.0g
K 2 HPO 4  solution...........................................................................3.0g
MgSO 4 ·7H 2 O solution..................................................................3.0g
CaCl 2  solution...............................................................................1.0g
NaCl solution................................................................................1.0g
FeCl 3 solution ............................................................................0.1mL
NaNO 3 Solution:
Composition per 400.0mL:
NaNO 3 .........................................................................................10.0g
Preparation of NaNO 3 Solution: Add NaNO 3  to distilled/deion-
ized water and bring volume to 400.0mL. Mix thoroughly.
CaCl 2 Solution:
Composition per 400.0mL:
CaCl 2 .............................................................................................1.0g
Preparation of CaCl 2 Solution: Add CaCl 2  to distilled/deionized
water and bring volume to 400.0mL. Mix thoroughly.
MgSO 4 ·7H 2 O Solution:
Composition per 400.0mL:
MgSO 4 ·7H 2 O................................................................................3.0g
Preparation of MgSO 4 ·7H 2 O Solution: Add MgSO 4 ·7H 2 O to
distilled/deionized water and bring volume to 400.0mL. Mix thorough-
ly.
K 2 HPO 4 Solution:
Composition per 400.0mL:
K 2 HPO 4 .........................................................................................3.0g
Preparation of K 2 HPO 4 Solution: Add K 2 HPO 4  to distilled/de-
ionized water and bring volume to 400.0mL. Mix thoroughly.
KH 2 PO 4 Solution:
Composition per 400.0mL:
KH 2 PO 4 .........................................................................................7.0g
Preparation of KH 2 PO 4 Solution: Add KH 2 PO 4  to distilled/de-
ionized water and bring volume to 400.0mL. Mix thoroughly.
NaCl Solution:
Composition per 400.0mL:
NaCl..............................................................................................1.0g
Preparation of NaCl Solution: Add NaCl to distilled/deionized
water and bring volume to 400.0mL. Mix thoroughly.
FeCl 3 Solution:
Composition per 100.0mL:
FeCl 3 .............................................................................................1.0g
Preparation of FeCl 3 Solution: Add FeCl 3  to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Bristol's Solution: Add 10.0mL of NaNO 3  solu-
tion, 10.0mL of CaCl 2  solution, 10.0mL of MgSO 4 ·7H 2 O solution,
10.0mL of NaNO 3  solution, 10.0mL of K 2 HPO 4  solution, 10.0mL of
KH 2 PO 4  solution, and 10.0mL of NaCl solution to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Add 0.1mL of FeCl 3
solution. Mix thoroughly.
Preparation of Medium: Add proteose peptone and agar to 1.0L of
Bristol’s solution. Mix thoroughly. Gently heat and bring to boiling. Dis-
tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation of Ankistrodesmus angustus, Ankistrodesmus
braunii, Botrydium becherianum, Botrydium cystosum, Botrydium stolo-
niferum, Bracteacoccus grandis, Bumilleria sicula, Characium polymor-
phum, Chlamydomonas species, Chlorella species, Chlorosphaera klebsii,
Coelastrum proboscideum, Crucigenia apiculata, Dictyochloris fragrans,
Dictyosphaerium ehrenbergianum, Dictyosphaerium pulchellum, Elaka-
tothrix viridis, Haematococcus lacustris, Interfilum paradoxum, Klebsor-
midium subtilissimum, Lobomonas piriformis, Mesotaenium caldariorum,
Mischococcus sphaerocephalus, Monodus subterraneus, Muriella auran-
tiaca, Muriella decolor, Nephrochlamys subsolitaria, Nephrodiella brevis,
Oocystis species, Ophiocytium majus, Pediastrum tetras, Polyedriella hel-
vetica, Protosiphon botryoides, Scenedesmus armatus, Scenedesmus com-
munis, Scenedesmus obliquus, Tetracystis disociata, Tribonema aequale,
Ulothrix gigas, Vitreochlamys incisa, and Vischeria punctata.