Molybdate Agar

[所属分类:培养基配方] [发布时间:2021-6-7] [发布人:网站管理员2] [阅读次数:] [返回]

Molybdate Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per101.5mL:

Base........................................................................................100.0mL
Phosphomolybdic acid solution.................................................1.5mL
pH 5.3 ± 0.2 at 25°C
Base:
Composition per liter:
Sucrose........................................................................................40.0g
Agar............................................................................................15.0g
Meat peptone ..............................................................................10.0g
Preparation of Base: Add components to distilled/deionized water
and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.6. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C.
Phosphomolybdic Acid Solution:
Composition per 100.0mL:
P 2 O 5 ·2OMoO 3 .............................................................................12.5g
Preparation of Base: Add P 2 O 5 ·2OMoO 3 (phospho-12-molybdic
acid, 12–molybdophosphoric acid, or PMA) to sterile distilled/deion-
ized water. Mix thoroughly. Do not adjust pH.
Preparation of Medium: To 100.0mL of cooled sterile base, add
1.5mL of phosphomolybdic acid solution. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and presumptive identification of yeast, especially
Candida species. Candida albicans appears as smooth, medium olive
colonies with medium olive bottoms. Candida stellatoidea appears as
shiny, light gray colonies with light gray bottoms. Candida tropicalis
appears as smooth, shiny, dark blue/gray colonies with dark blue/gray
bottoms. Candida krusei appears as smooth, dull white colonies with
white bottoms. Saccharomyces cerevisiae appears as smooth, shiny light
blue/dark blue colonies with dark blue/green bottoms.