Monsur Agar (Taurocholate Tellurite Gelatin Agar)

[所属分类:培养基配方] [发布时间:2021-6-7] [发布人:网站管理员2] [阅读次数:] [返回]

Monsur Agar
(Taurocholate Tellurite Gelatin Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Gelatin.........................................................................................30.0g
Agar ............................................................................................15.0g
Casein peptone............................................................................10.0g
NaCl............................................................................................10.0g
Sodium taurocholate.....................................................................5.0g
Na 2 CO 3 ·H 2 O.................................................................................1.0g
K 2 TeO 3 solution.......................................................................10.0mL
pH 8.5 ± 0.2 at 25°C
K 2 TeO 3 Solution:
Composition per 10.0mL:
K 2 TeO 3 ........................................................................................0.02g
Preparation of K 2 TeO 3 Solution: Add K 2 TeO 3 to 10.0mL of dis-
tilled/deionized water. Mix thoroughly. Filter sterilize.
Caution: Potassium tellurite is toxic.
Preparation of Medium: Add components, except K 2 TeO 3 solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Add 10.0mL of sterile
K 2 TeO 3 solution. Mix thoroughly. Pour into sterile Petri dishes or dis-
tribute into sterile tubes.
Use: For the isolation of Vibrio cholerae from fecal specimens.