MOF HiVeg Medium with Carbohydrate

[所属分类:培养基配方] [发布时间:2021-6-7] [发布人:网站管理员2] [阅读次数:] [返回]

MOF HiVeg Medium with Carbohydrate

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..............................................................................................9.7g
MnCl 2 ............................................................................................4.4g
Agar..............................................................................................3.0g
Na 2 SO 4 ..........................................................................................1.6g
Plant hydrolysate ..........................................................................1.0g
CaCl 2 .............................................................................................0.9g
(NH 4 ) 2 SO 4 ....................................................................................0.5g
Tris hydroxymethyl aminomethane..............................................0.5g
KCl............................................................................................0.275g
Yeast extract..................................................................................0.1g
NaHCO 3 ......................................................................................0.08g
KBr .............................................................................................0.04g
SrCl 2 .........................................................................................0.017g
H 3 BO 3 ....................................................................................... 0.011g
Phenol red...................................................................................0.01g
Na 2 HPO 4 ....................................................................................4.0mg
Sodium silicate...........................................................................2.0mg
NaFl ...........................................................................................1.2mg
NH 4 NO 3 .....................................................................................0.8mg
Carbohydrate solution............................................................100.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate...............................................................................10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL. Adonitol, ara-
binose, cellobiose, glucose, dulcitol, fructose, galactose, inositol, lac-
tose, maltose, mannitol, raffinose, rhamnose, salicin, sorbitol, sucrose,
trehalose, xylose, or other carbohydrates may be used. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Gently heat until dissolved. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 50°C. Aseptically add 100.0mL sterile
carbohydrate soltuion. Mix thoroughly. Aseptically distribute into
screw-capped tubes in 5.0mL volumes.
Use: For the differentiation of marine bacteria on the basis of fermen-
tative and oxidative metabolism of carbohydrates.