Moeller KCN Broth Base

[所属分类:培养基配方] [发布时间:2021-6-7] [发布人:网站管理员2] [阅读次数:] [返回]

Moeller KCN Broth Base

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 .....................................................................................5.64g
NaCl..............................................................................................5.0g
Pancreatic digest of casein............................................................1.5g
Peptic digest of animal tissue........................................................1.5g
KH 2 PO 4 .....................................................................................0.225g
KCN solution...........................................................................0.15mL
pH 7.6 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
KCN Solution:
Composition per 100.0mL:
KCN..............................................................................................0.5g
Preparation of KCN Solution:  Add KCN to 100.0mL of cold dis-
tilled/deionized water. Mix thoroughly and cap. Do not mouth pipette.
Caution: Cyanide is toxic.
Preparation of Medium:  Add components, except KCN solution, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Prior to use, add 0.15mL of KCN solution. Mix thoroughly. Aseptically
distribute into sterile tubes.
Use: For the differentiation of Gram-negative enteric bacteria on the
basis of their ability to grow in the presence of cyanide.