Modified TH Agar (DSMZ Medium 1061)

[所属分类:培养基配方] [发布时间:2021-6-4] [发布人:网站管理员2] [阅读次数:] [返回]

Modified TH Agar
(DSMZ Medium 1061)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ...........................................................................................15.0g
Tryptone .......................................................................................5.0g
NaCl .............................................................................................5.0g
Yeast extract .................................................................................3.0g
MnSO 4 ·2H 2 O..............................................................................0.01g
Trace elements solution SL-6....................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O .................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5.
Gently heat while stirring and bring to boiling. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into Petri
dishes or leave in tubes.
Use: For the cultivation of Anoxybacillus voinovskiensis.