Modified Thermus Medium (DSMZ Medium 630)

[所属分类:培养基配方] [发布时间:2021-6-4] [发布人:网站管理员2] [阅读次数:] [返回]

Modified Thermus Medium
(DSMZ Medium 630)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................28.0g
Yeast extract..................................................................................2.5g
Tryptone........................................................................................2.5g
MgCl 2 ·6H 2 O ..........................................................................200.0mg
Nitrilotriacetic acid................................................................100.0mg
CaSO 4 ·2H 2 O............................................................................40.0mg
Phosphate solution.................................................................100.0mL
Ferric citrate solution.................................................................0.5mL
Trace elements solution .............................................................0.5mL
pH 7.2 ± 0.2 at 25°C
Phosphate Solution:
Composition per liter:
Na 2 HPO 4 ·12H 2 O.........................................................................43.0g
KH 2 PO 4 .......................................................................................5.44g
Preparation of Phosphate Solution:  Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad-
just pH to 7.2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C.
Ferric Citrate Solution:
Composition per liter:
Ferric citrate..................................................................................2.5g
Preparation of Ferric Citrate Solution:  Add ferric citrate to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Trace Elements Solution:
Composition per liter:
Nitrilotriacetic acid.....................................................................12.8g
FeCl 2 ·4H 2 O...................................................................................1.0g
MnCl 2 ·4H 2 O..................................................................................0.5g
CoCl 2 ·4H 2 O ..................................................................................0.3g
Na 2 MoO 4 ·4H 2 O .......................................................................50.0mg
CuCl 2 ·2H 2 O .............................................................................50.0mg
NiCl 2 ·6H 2 O..............................................................................20.0mg
H 3 BO 3 ......................................................................................20.0mg
Preparation of Trace Elements Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 7.0 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L. Mix thoroughly. Adjust pH to 6.8.
Preparation of Medium: Add components, except phosphate solu-
tion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C. Aseptically add 100.0mL phosphate solution. Mix thoroughly.
Adjust pH to 7.2. Pour into Petri dishes or aseptically distribute into ster-
ile tubes.
Use:  For the cultivation and maintenance of Thermus sp., Rhodothermus
marinus, and Albidovulum inexpectatum.