Modified Soybean HiVeg Agar

[所属分类:培养基配方] [发布时间:2021-6-4] [发布人:网站管理员2] [阅读次数:] [返回]

Modified Soybean HiVeg Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Plant hydrolysate.........................................................................17.0g
Agar ............................................................................................15.0g
NaCl..............................................................................................5.0g
Papaic digest of soyabean meal....................................................3.0g
K 2 HPO 4 .........................................................................................2.5g
Glucose.........................................................................................2.5g
Polysorbate 80 .........................................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium, without polysorbate 80, is available as a pre-
mixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the microbiological assay of polymyxin B, colistin sulfate,
and sodium colistimethate.