Modified Soyabean Bile Broth Base

[所属分类:培养基配方] [发布时间:2021-6-4] [发布人:网站管理员2] [阅读次数:] [返回]

Modified Soyabean Bile Broth Base

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Casein enzymic hydrolysate .......................................................17.0g
NaCl..............................................................................................5.0g
K 2 HPO 4 .........................................................................................4.0g
Papaic digest of soybean meal......................................................3.0g
D -Glucose......................................................................................2.5g
Bile salts mixture..........................................................................1.5g
Selective supplement solution .................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Selective Supplement Solution:
Composition per 10.0mL:
Novobiocin...............................................................................10.0mg
Preparation of Selective Supplement Solution:  Add novobio-
cin to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except selective sup-
plement solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add selective supplement solution.
Mix thoroughly. Pour into Petri dishes or aseptically distribute into
sterile tubes.
Use: For the detection of Escherichia coli O157:H7 from food.