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培养基配方
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Modified Oxford Listeria Selective Agar (Oxford Agar, Modified) (Listeria Selective Agar, Modified Oxford) (MOX Agar) (BAM M103a)
[所属分类:培养基配方] [发布时间:2021-6-4] [发布人:网站管理员2] [阅读次数:] [返回]
Modified Oxford Listeria Selective Agar
(Oxford Agar, Modified)
(Listeria Selective Agar, Modified Oxford)
(MOX Agar)
(BAM M103a)
LiCl.............................................................................................15.0g
Agar............................................................................................12.0g
NaCl..............................................................................................5.0g
Cornstarch.....................................................................................1.0g
Esculin ..........................................................................................1.0g
Ferric ammonium citrate...............................................................0.5g
Buffered colistin methane sulfonate solution............................1.0mL
Buffered moxalactam solution...................................................2.0mL
pH 7.2 ± 0.2 at 25°C
Buffered Colistin Methane Sulfonate
Solution:
Composition per 100.0mL:
Colistin methane sulfonate ...........................................................1.0g
Potassium phosphate buffer, 0.1M. pH 6.0............................100.0mL
Preparation of Buffered Colistin Methane Sulfonate Solu-
tion: Add colistin methane sulfonate to 100.0mL 0.1M potassium
phosphate buffer. Mix thoroughly. Adjust pH to 6.0. Filter sterilize.
Store at –20°C.
Buffered Moxalactam Solution:
Composition per 100.0mL:
Sodium or ammonium moxalactam..............................................1.0g
Preparation of Buffered Moxalactam Solution: Add sodium or
ammonium moxalactam to distilled/deionized water and bring volume
to 100.0mL. Mix thoroughly. Filter sterilize. Store at –20°C.
Preparation of Medium: Gradually add components, except buff-
ered moxalactam solution, to distilled/deionized water and bring vol-
ume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Gently heat and bring
to boiling. Autoclave for 10 min at 15 psi pressure–121°C. Cool quick-
ly to 46°C. Aseptically add 2.0mL of sterile moxalactam solution. Mix
thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use : For the isolation and cultivation of Listeria monocytogenes from
specimens containing a mixed bacterial flora.
(Oxford Agar, Modified)
(Listeria Selective Agar, Modified Oxford)
(MOX Agar)
(BAM M103a)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per 1002.0mL:
Special peptone...........................................................................23.0gLiCl.............................................................................................15.0g
Agar............................................................................................12.0g
NaCl..............................................................................................5.0g
Cornstarch.....................................................................................1.0g
Esculin ..........................................................................................1.0g
Ferric ammonium citrate...............................................................0.5g
Buffered colistin methane sulfonate solution............................1.0mL
Buffered moxalactam solution...................................................2.0mL
pH 7.2 ± 0.2 at 25°C
Buffered Colistin Methane Sulfonate
Solution:
Composition per 100.0mL:
Colistin methane sulfonate ...........................................................1.0g
Potassium phosphate buffer, 0.1M. pH 6.0............................100.0mL
Preparation of Buffered Colistin Methane Sulfonate Solu-
tion: Add colistin methane sulfonate to 100.0mL 0.1M potassium
phosphate buffer. Mix thoroughly. Adjust pH to 6.0. Filter sterilize.
Store at –20°C.
Buffered Moxalactam Solution:
Composition per 100.0mL:
Sodium or ammonium moxalactam..............................................1.0g
Preparation of Buffered Moxalactam Solution: Add sodium or
ammonium moxalactam to distilled/deionized water and bring volume
to 100.0mL. Mix thoroughly. Filter sterilize. Store at –20°C.
Preparation of Medium: Gradually add components, except buff-
ered moxalactam solution, to distilled/deionized water and bring vol-
ume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Gently heat and bring
to boiling. Autoclave for 10 min at 15 psi pressure–121°C. Cool quick-
ly to 46°C. Aseptically add 2.0mL of sterile moxalactam solution. Mix
thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use : For the isolation and cultivation of Listeria monocytogenes from
specimens containing a mixed bacterial flora.
