Modified PYNFH Medium

[所属分类:培养基配方] [发布时间:2021-6-4] [发布人:网站管理员2] [阅读次数:] [返回]

Modified PYNFH Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone........................................................................................10.0g
Yeast extract................................................................................10.0g
Yeast nucleic acid..........................................................................1.0g
Folic acid..................................................................................15.0mg
Hemin.........................................................................................1.0mg
Fetal bovine serum, heat inactivated......................................100.0mL
Buffer solution.........................................................................20.0mL
pH 6.5 ± 0.2 at 25°C
Buffer Solution:
Composition per liter:
Na 2 HPO 4 .....................................................................................25.0g
KH 2 PO 4 .......................................................................................18.1g
Preparation of Buffer Solution: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to
6.5. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Add components, except buffer solution
and fetal bovine serum, to distilled/deionized water and bring volume
to 880.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 25°C. Aseptically add 20.0mL of sterile buffer solution
and 100.0mL of sterile, heat-inactivated fetal bovine serum. Mix thor-
oughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Dexiostoma campyla, Hartmannella vermi-
formis, Naegleria australiensis, Naegleria fowleri, Naegleria gruberi,
Naegleria jadini, Phytomonas davidi, Tetrahymena species, Vahl-
kampfia avara, and Willaertia magna.