Modified MYP HiVeg Agar Base with Egg Yok and Polymyxin B

[所属分类:培养基配方] [发布时间:2021-6-4] [发布人:网站管理员2] [阅读次数:] [返回]

Modified MYP HiVeg Agar Base
with Egg Yok and Polymyxin B

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................12.0g
D -Mannitol..................................................................................10.0g
Plant peptone...............................................................................10.0g
NaCl............................................................................................10.0g
Plant extract No. 1 ........................................................................1.0g
Phenol red.................................................................................0.025g
Egg yolk emulsion, 20%..........................................................10.0mL
Polymyxin B solution ................................................................1.0mL
pH 7.1 ± 0.2 at 25°C
Source: This medium, without egg yolk emulsion and polymyxin B
solution, is available as a premixed powder from HiMedia.
Egg Yolk Emulsion, 20%:
Composition per 100.0mL:
Chicken egg yolks............................................................................11
Whole chicken egg.............................................................................1

NaCl (0.9% solution)...............................................................80.0mL

Preparation of Egg Yolk Emulsion, 20%: Soak eggs with 1:100
dilution of saturated mercuric chloride solution for 1 min. Crack eggs
and separate yolks from whites. Mix egg yolks with 1 chicken egg.
Measure 20.0mL of egg yolk emulsion and add to 80.0mL of 0.9%
NaCl solution. Mix thoroughly. Filter sterilize. Warm to 45°–50°C.
Polymyxin B Solution:
Composition per 1.0mL:
Polymyxin B..............................................................................1.0mg
Preparation of Polymyxin B Solution: Add polymyxin B to dis-
tilled/deionized water and bring volume to 1.0mL. Mix thoroughly. Fil-
ter sterilize.
Preparation of Medium: Add components—except egg yolk
emulsion, 20%, and polymyxin B solution—to distilled/deionized wa-
ter and bring volume to 989.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add 10.0mL of sterile egg yolk emulsion,
20%, and 1.0mL of sterile polymyxin B solution. Mix thoroughly. Pour
into sterile Petri dishes.
Use: For the cultivation and maintenance of Bacillus cereus. For the
isolation and identification of Bacillus species and pathogenic staphy-
lococci.