Modified MYP Agar Base

[所属分类:培养基配方] [发布时间:2021-6-4] [发布人:网站管理员2] [阅读次数:] [返回]

Modified MYP Agar Base

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................12.0g
Peptic digest of animal tissue......................................................10.0g
D -Mannitol..................................................................................10.0g
NaCl............................................................................................10.0g
Meat extract ..................................................................................1.0g
Phenol Red................................................................................0.025g
Egg yolk emulsion.................................................................100.0mL
Selective supplement solution .................................................10.0mL
pH 7.1 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Egg Yolk Emulsion
Composition per 100.0mL:
Chicken egg yolks............................................................................11
Whole chicken egg.............................................................................1
NaCl (0.9% solution)...............................................................50.0mL
Preparation of Egg Yolk Emulsion: Soak eggs with 1:100 dilu-
tion of saturated mercuric chloride solution for 1 min. Crack eggs and
separate yolks from whites. Mix egg yolks with 1 chicken egg. Beat to
form emulsion. Measure 50.0mL of egg yolk emulsion and add to
50.0mL of 0.9% NaCl solution. Mix thoroughly. Filter sterilize. Warm
to 45°–50°C.
Selective Supplement Solution:
Composition per 10.0mL:
Polymyxin B.............................................................................1000U
Preparation of Selective Supplement Solution:  Add polymyx-
in B to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except egg yolk emul-
sion and selective supplement solution, to distilled/deionized water and
bring volume to 890.0mL. Mix thoroughly. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 50°C. Aseptically add egg yolk emulsion
and selective supplement solution. Mix thoroughly. Pour into Petri
dishes or aseptically distribute into sterile tubes.
Use: For the isolation and identification of Bacillus species and patho-
genic staphylococci.