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Modified CPLM HiVeg Medium Base with Horse Serum (Trichomonas Modified CPLM HiVeg Medium Base)

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

Modified CPLM HiVeg Medium Base
with Horse Serum
(Trichomonas Modified CPLM HiVeg Medium Base)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Plant peptone ..............................................................................32.0g
Liver digest.................................................................................20.0g
L -Cysteine·HCl .............................................................................2.4g
Maltose .........................................................................................1.6g
Ringer's salt solution, 1/4X...........................................................1.0L
Horse serum...........................................................................100.0mL
pH 6.0 ± 0.2 at 25°C
Ringer's Salt Solution, 1/4X:
Composition per 400.0mL:
NaCl..............................................................................................9.0g
KCl............................................................................................0.042g
CaCl 2 .........................................................................................0.024g
Preparation of Ringer's Salt Solution, 1/4X : Add components
to distilled/deionized water and bring volume to 400.0mL. Mix thor-
oughly.
Preparation of Medium: Add components, except horse serum, to
1.0L Ringer’s salt solution, 1/4X. Mix thoroughly. Adjust pH to 6.0.
Gently heat and bring to boiling. Autoclave for 10 min at 15 psi pres-
sure–121°C. Cool to 25°C. Aseptically add 100.0mL of sterile, heat-in-
activated horse serum. Mix thoroughly. Aseptically distribute into
sterile, screw-capped tubes or flasks.
Use: For the cultivation of Trichomonas vaginalis.

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Modified CPLM HiVeg Medium Base with Horse Serum (Trichomonas Modified CPLM HiVeg Medium Base)