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Modified CPLM HiVeg Medium Base with Horse Serum, Penicillin, Streptomycin, and Nystatin (Trichomonas Modified CPLM HiVeg Medium Base)
[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]
Modified CPLM HiVeg Medium Base with
Horse Serum, Penicillin, Streptomycin, and Nystatin
(Trichomonas Modified CPLM HiVeg Medium Base)
Liver digest.................................................................................20.0g
L -Cysteine·HCl .............................................................................2.4g
Maltose .........................................................................................1.6g
Ringer's salt solution, 1/4X...........................................................1.0L
Horse serum...........................................................................100.0mL
Penicllin-streptomycin solution...............................................10.0mL
Nystatin solution......................................................................10.0mL
pH 6.0 ± 0.2 at 25°C
Ringer's Salt Solution, 1/4X:
Composition per 400.0mL:
NaCl..............................................................................................9.0g
KCl............................................................................................0.042g
CaCl 2 .........................................................................................0.024g
Preparation of Ringer's Salt Solution, 1/4X : Add components
to distilled/deionized water and bring volume to 400.0mL. Mix thor-
oughly.
Penicllin-Streptomycin Solution:
Composition per 10.0mL:
Streptomycin.................................................................................0.1g
Penicillin...........................................................................1,000,000U
Preparation of Penicllin-Streptomycin Solution : Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize
Nystatin Solution:
Composition per 10.0mL:
Nystatin..................................................................................50,000U
Preparation of Nystatin Solution : Add nystatin to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize
Preparation of Medium: Add components, except horse serum,
penicillin-streptomycin solution, and nystatin solution, to 1.0L of
Ringer’s salt solution, 1/4X. Mix thoroughly. Adjust pH to 6.0. Gently
heat and bring to boiling. Autoclave for 10 min at 15 psi pressure–
121°C. Cool to 25°C. Aseptically add 100.0mL of sterile, heat-inacti-
vated horse serum, 10.0mL sterile penicllin-streptomycin solution, and
10.0mL sterile nystatin solution. . Mix thoroughly. Aseptically distrib-
ute into sterile, screw-capped tubes or flasks.
Use: For the selective cultivation of Trichomonas vaginalis.
Horse Serum, Penicillin, Streptomycin, and Nystatin
(Trichomonas Modified CPLM HiVeg Medium Base)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Plant peptone ..............................................................................32.0gLiver digest.................................................................................20.0g
L -Cysteine·HCl .............................................................................2.4g
Maltose .........................................................................................1.6g
Ringer's salt solution, 1/4X...........................................................1.0L
Horse serum...........................................................................100.0mL
Penicllin-streptomycin solution...............................................10.0mL
Nystatin solution......................................................................10.0mL
pH 6.0 ± 0.2 at 25°C
Ringer's Salt Solution, 1/4X:
Composition per 400.0mL:
NaCl..............................................................................................9.0g
KCl............................................................................................0.042g
CaCl 2 .........................................................................................0.024g
Preparation of Ringer's Salt Solution, 1/4X : Add components
to distilled/deionized water and bring volume to 400.0mL. Mix thor-
oughly.
Penicllin-Streptomycin Solution:
Composition per 10.0mL:
Streptomycin.................................................................................0.1g
Penicillin...........................................................................1,000,000U
Preparation of Penicllin-Streptomycin Solution : Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize
Nystatin Solution:
Composition per 10.0mL:
Nystatin..................................................................................50,000U
Preparation of Nystatin Solution : Add nystatin to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize
Preparation of Medium: Add components, except horse serum,
penicillin-streptomycin solution, and nystatin solution, to 1.0L of
Ringer’s salt solution, 1/4X. Mix thoroughly. Adjust pH to 6.0. Gently
heat and bring to boiling. Autoclave for 10 min at 15 psi pressure–
121°C. Cool to 25°C. Aseptically add 100.0mL of sterile, heat-inacti-
vated horse serum, 10.0mL sterile penicllin-streptomycin solution, and
10.0mL sterile nystatin solution. . Mix thoroughly. Aseptically distrib-
ute into sterile, screw-capped tubes or flasks.
Use: For the selective cultivation of Trichomonas vaginalis.
