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Modified Campylobacter Blood-Free Selective Agar Base (Modified Campylobacter Charcoal Differential Agar) (Modified CCDA) (BAM M30a)
[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]
Modified Campylobacter Blood-Free
Selective Agar Base
(Modified Campylobacter Charcoal Differential Agar)
(Modified CCDA)
(BAM M30a)
Beef extract.................................................................................10.0g
Peptone........................................................................................10.0g
NaCl..............................................................................................5.0g
Charcoal........................................................................................4.0g
Casein hydrolysate........................................................................3.0g
Yeast extract..................................................................................2.0g
Sodium deoxycholate....................................................................1.0g
FeSO 4 ..........................................................................................0.25g
Sodium pyruvate.........................................................................0.25g
Cefoperazone solution ...............................................................4.0mL
Amphotericin B solution............................................................4.0mL
Rifampicin solution....................................................................4.0mL
pH 7.4 ± 0.2 at 25°C
Cefoperazone Solution:
Composition per 10.0mL:
Cefoperazone............................................................................0.037g
Preparation of Cefoperazone Solution: Add cefoperazone to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Rifampicin Solution:
Composition per 100.0mL:
Rifampicin ..................................................................................0.25g
Ethanol, absolute......................................................................50.0mL
Preparation of Rifampicin Solution: Add rifampicin to 50.0mL
of ethanol. Mix thoroughly. Bring volume to 100.0mL with distilled/
deionized water. Filter sterilize.
Amphotericin B Solution:
Composition per 10.0mL:
Amphotericin B.........................................................................0.005g
Preparation of Amphotericin B Solution: Add amphotericin B
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize. Can be stored for 1 year at –20°C.
Preparation of Medium: Add components, except cefoperazone
solution, amphotericin B solution, and rifampicin solution, to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile cefopera-
zone solution, 10.0mL of sterile amphotericin B solution, and 10.0mL
of sterile rifampicin solution. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use : For the cultivation of Campylobacter species. For the recovery of
injured Campylobacter spp. from foods.
Selective Agar Base
(Modified Campylobacter Charcoal Differential Agar)
(Modified CCDA)
(BAM M30a)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per 1012.0mL:
Agar ............................................................................................12.0gBeef extract.................................................................................10.0g
Peptone........................................................................................10.0g
NaCl..............................................................................................5.0g
Charcoal........................................................................................4.0g
Casein hydrolysate........................................................................3.0g
Yeast extract..................................................................................2.0g
Sodium deoxycholate....................................................................1.0g
FeSO 4 ..........................................................................................0.25g
Sodium pyruvate.........................................................................0.25g
Cefoperazone solution ...............................................................4.0mL
Amphotericin B solution............................................................4.0mL
Rifampicin solution....................................................................4.0mL
pH 7.4 ± 0.2 at 25°C
Cefoperazone Solution:
Composition per 10.0mL:
Cefoperazone............................................................................0.037g
Preparation of Cefoperazone Solution: Add cefoperazone to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Rifampicin Solution:
Composition per 100.0mL:
Rifampicin ..................................................................................0.25g
Ethanol, absolute......................................................................50.0mL
Preparation of Rifampicin Solution: Add rifampicin to 50.0mL
of ethanol. Mix thoroughly. Bring volume to 100.0mL with distilled/
deionized water. Filter sterilize.
Amphotericin B Solution:
Composition per 10.0mL:
Amphotericin B.........................................................................0.005g
Preparation of Amphotericin B Solution: Add amphotericin B
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize. Can be stored for 1 year at –20°C.
Preparation of Medium: Add components, except cefoperazone
solution, amphotericin B solution, and rifampicin solution, to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile cefopera-
zone solution, 10.0mL of sterile amphotericin B solution, and 10.0mL
of sterile rifampicin solution. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use : For the cultivation of Campylobacter species. For the recovery of
injured Campylobacter spp. from foods.
