Modified Buffered Charcoal HiVeg Agar Base with Cysteine

[所属分类:培养基配方] [发布时间:2021-6-3] [发布人:网站管理员2] [阅读次数:] [返回]

Modified Buffered Charcoal HiVeg Agar Base
with Cysteine

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................17.0g
ACES buffer ...............................................................................10.0g
Plant peptone No. 3.....................................................................10.0g
Charcoal, activated .......................................................................2.0g
α-Ketoglutarate monopotassium salt............................................1.0g
L -Cysteine solution....................................................................4.0mL
pH 6.9 ± 0.2 at 25°C
Source: This medium, without  L -cysteine solution, is available as a
premixed powder from HiMedia.
L -Cysteine Solution:
Composition per 10.0mL:
L- cysteine·HCl·H 2 O ......................................................................0.4g
L -Cysteine Solution: Add  L- cysteine·HCl·H 2 O to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Preparation of Medium:  Add components, except  L- cysteine solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Adjust medium to pH 6.9 with 1N KOH. Heat gently and bring
to boiling for 1 min. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 50°–55°C. Aseptically add 4.0mL of  L- cysteine solution. Mix
thoroughly. Pour into sterile Petri dishes with constant agitation to keep
charcoal in suspension.
Use: For the isolation, cultivation, and maintenance of Legionella
pneumophila and other Legionella species from environmental and
clinical specimens.