Minerals Modified Glutamate Agar

[所属分类:培养基配方] [发布时间:2021-6-2] [发布人:网站管理员2] [阅读次数:] [返回]

Minerals Modified Glutamate Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Lactose........................................................................................10.0g
Sodium glutamate.......................................................................6.35g
NH 4 Cl ...........................................................................................2.5g
K 2 HPO 4 .........................................................................................0.9g
Sodium formate...........................................................................0.25g
MgSO 4 ·7H 2 O................................................................................0.1g
L -Aspartic acid..........................................................................0.024g
L -Arginine...................................................................................0.02g
L -Cystine.....................................................................................0.02g
Bromcresol Purple ......................................................................0.01g
CaCl 2 ·2H 2 O.................................................................................0.01g
Ferric ammonium citrate.............................................................0.01g
Nicotinic acid.............................................................................1.0mg
Pantothenic acid.........................................................................1.0mg
Thiamine....................................................................................1.0mg
pH 6.7 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 10 min at 11
psi pressure–116°C. Pour into sterile Petri dishes in 20.0mL volumes.
Use: For the cultivation of coliform bacteria from foods.