Minerals Modified Medium

[所属分类:培养基配方] [发布时间:2021-6-2] [发布人:网站管理员2] [阅读次数:] [返回]

Minerals Modified Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Lactose........................................................................................20.0g
Sodium glutamate.......................................................................12.7g
NH 4 Cl ...........................................................................................5.0g
K 2 HPO 4 .........................................................................................1.8g
Sodium formate ............................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
L- Aspartic acid..........................................................................0.048g
L- Cystine.....................................................................................0.04g
L- Arginine...................................................................................0.04g
Ferric ammonium citrate.............................................................0.02g
CaCl 2 ·2H 2 O ................................................................................0.02g
Bromcresol Purple......................................................................0.02g
Thiamine....................................................................................2.0mg
Nicotinic acid.............................................................................2.0mg
Pantothenic acid.........................................................................2.0mg
pH 6.7 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add NH 4 Cl to distilled/deionized water
and bring volume to 800.0mL. Add remaining components and bring
volume to 1.0L. Mix thoroughly. Adjust pH to 6.7. Distribute into tubes
or flasks. Autoclave for 10 min at 10 psi pressure–116°C. Check pH af-
ter autoclaving. This medium is double strength.
Use: For the enumeration of coliform bacteria in water.