Mineral Salts for Thermophiles (L Salts for Thermophiles)

[所属分类:培养基配方] [发布时间:2021-6-2] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Salts for Thermophiles
(L Salts for Thermophiles)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaNO 3 .........................................................................................0.25g
NH 4 Cl .........................................................................................0.25g
Na 2 HPO 4 .....................................................................................0.21g
MgSO 4 ·7H 2 O................................................................................0.2g
NaH 2 PO 4 .....................................................................................0.09g
KCl..............................................................................................0.04g
CaCl 2 ...........................................................................................0.02g
FeSO 4 .........................................................................................1.0mg
Trace minerals solution............................................................10.0mL
n-Heptadecane ...........................................................................1.0mL
Trace Minerals Solution:
Composition per liter:
ZnSO 4 ·7H 2 O..............................................................................7.0mg
H 3 BO 4 ........................................................................................1.0mg
MoO 3 ..........................................................................................1.0mg
CuSO 4 ·5H 2 O...........................................................................500.0μg
CoSO 4 ·7H 2 O.............................................................................18.0μg
MnSO 4 ·5H 2 O..............................................................................7.0μg
Preparation of Trace Minerals Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except n-heptadecane,
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add the n-
heptadecane. Mix thoroughly. Distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Bacillus thermoleo-
vorans.