Mineral Salts Peptonized Milk Agar (SPMA)

[所属分类:培养基配方] [发布时间:2021-6-2] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Salts Peptonized Milk Agar
(SPMA)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Milk, peptonized...........................................................................1.0g
Mineral solution.....................................................................100.0mL
Mineral Solution:
Composition per 100.0mL:
MgSO 4 ·7H 2 O................................................................................0.5g
CaCl 2 ...........................................................................................0.25g
K 2 HPO 4 .......................................................................................0.25g
(NH 4 ) 2 SO 4 ....................................................................................0.1g
FeCl 3 ·6H 2 O.................................................................................0.01g
MnCl 2 .........................................................................................0.1mg
Preparation of Mineral Solution: Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except mineral solu-
tion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL of
sterile mineral solution. Mix thoroughly. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use: For the cultivation of freshwater Myxobacterium species.