Mineral Salts Medium for Thermophiles

[所属分类:培养基配方] [发布时间:2021-6-2] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Salts Medium for Thermophiles

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaNO 3 ........................................................................................0.25g
NH 4 Cl .........................................................................................0.25g
Na 2 HPO 4 ................................................................................210.0mg
MgSO 4 ·7H 2 O.........................................................................200.0mg
NaH 2 PO 4 ..................................................................................90.0mg
KCl...........................................................................................40.0mg
CaCl 2 ........................................................................................15.0mg
FeSO 4 .........................................................................................1.0mg
Trace minerals solution............................................................10.0mL
n-Heptadecane ...........................................................................1.0mL
Trace Minerals Solution:
Composition per liter:
ZnSO 4 ·7H 2 O..............................................................................7.0mg
H 3 BO 4 ........................................................................................1.0mg
MoO 3 .........................................................................................1.0mg
CuSO 4 ·5H 2 O...........................................................................500.0μg
CoSO 4 ·7H 2 O.............................................................................18.0μg
MnSO 4 ·5H 2 O..............................................................................7.0μg
Preparation of Trace Minerals Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Bacillus thermoleovorans.