Mineral Salts Medium with Methanol and Yeast Extract

[所属分类:培养基配方] [发布时间:2021-6-2] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Salts Medium with Methanol
and Yeast Extract

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaNH 4 HPO 4 ·4H 2 O.....................................................................1.74g
NaH 2 PO 4 ·H 2 O.............................................................................0.54g
MgSO 4 ·7H 2 O................................................................................0.2g
Yeast extract..................................................................................0.2g
KCl..............................................................................................0.04g
FeSO 4 ·7H 2 O...............................................................................5.0mg
Methanol....................................................................................5.0mL
Trace minerals solution..............................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Trace Minerals Solution:
Composition per liter:
H 3 BO 3 .........................................................................................2.86g
MnCl 2 ·4H 2 O................................................................................1.81g
ZnSO 4 ·7H 2 O...............................................................................0.22g
CuSO 4 ·5H 2 O...............................................................................0.08g
CoCl 2 · 6H 2 O.................................................................................0.06g
Na 2 MoO 4 ·2H 2 O .......................................................................25.0mg
Preparation of Trace Minerals Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except methanol, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Filter
sterilize methanol. Aseptically add sterile methanol to cooled, sterile
basal medium. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Pseudomonas species.