Agrobacterium Medium

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Agrobacterium Medium
Composition per liter:
Agar............................................................................................20.0g
Mannitol......................................................................................10.0g
NaNO 3 ..........................................................................................4.0g
MgCl 2 ............................................................................................2.0g

Calcium propionate.......................................................................1.2g
Mg 3 (PO 4 ) 2 .....................................................................................0.2g
MgSO 4 ..........................................................................................0.1g
MgCO 3 ......................................................................................0.075g
NaHCO 3 ....................................................................................0.075g
Supplement ............................................................................100.0mL
pH 7.1 ± 0.2 at 25°C
Supplement:
Composition per 100.0mL:
Berberine...................................................................................0.275g
Cycloheximide..............................................................................0.2g
Bacitracin......................................................................................0.1g
Na 2 SeO 3 ........................................................................................0.1g
Penicillin G.................................................................................0.06g
Streptomycin sulfate ...................................................................0.03g
Tyrothricin..................................................................................1.0mg
Preparation of Supplement: Add components to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except supplement, to
distilled/deionized water and bring volume to 900.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add 100.0mL of sterile supplement.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the selective isolation and cultivation of Agrobacterium spe-
cies.