Agrobacterium Medium

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Agrobacterium Medium
Composition per liter:
Agar ............................................................................................12.0g
Lactose..........................................................................................5.0g
Na 2 HPO 4 .......................................................................................1.8g
KNO 3 ............................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.1g
Supplement ............................................................................100.0mL
pH 6.8 ± 0.2 at 25°C
Supplement:
Composition per 100.0mL:
MnSO 4 ·4H 2 O..............................................................................3.35g
Ferric EDTA...............................................................................2.5mg
Preparation of Supplement: Add components to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except supplement, to
distilled/deionized water and bring volume to 900.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 1 min at 25 psi pressure–
130°C. Cool to 45°–50°C. Aseptically add sterile supplement. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the selective isolation and cultivation of Agrobacterium spe-
cies.